![]() Regarding the first point, the introduction of nonlinear microscopies such as two- and three-photon excitation fluorescence 1, 2, 3, 4 and harmonic generation imaging 5, 6, 7, 8, 9, 10 represent major improvements. Besides endoscopic approaches, two major strategies can be identified which have proven successful in enhancing image fidelity: i) the use of longer wavelengths to decrease scattering and ii) the use of adaptive optics to counteract aberrations. A serious obstacle for obtaining clear vision is the tissue itself, which is highly scattering due to its often inhomogeneous structure, and thus ultimately limits the achievable imaging depth. Microscopic imaging inside living tissue is a key technology for understanding the functioning of life at the cellular level and its organization to form entire organs. Using DASH, we demonstrate two-photon fluorescence imaging of microglia cells in highly turbid mouse hippocampal tissue down to a depth of 530 μm. This leads to rapid improvement of the wavefront correction, forming a focus after just one measurement iteration and achieving an order of magnitude higher signal enhancement at this stage than the previous state-of-the-art. Here, we introduce a quickly converging algorithm for non-invasive scattering compensation, termed DASH, in which holographic phase stepping interferometry enables new phase information to be updated after each measurement. However, fast, non-invasive determination of the required wavefront compensation remains challenging. Progress toward overcoming the distortions caused by scattering in turbid media has been made by shaping the excitation wavefront to redirect power into a single point in the imaging plane. ![]() Also what I dont get is in say the 64-5 you have all the other settings say A through L It seems like you can fine tune things with those settings but when you leave the sim and then go back into it the settings seem to revert to where they were,so I dont get what they are used for.Scattering in biological tissues is a major barrier for in vivo optical imaging of all but the most superficial structures. I went to a couple of sharp classes and none of the training goes through any of this stuff. I have mainly used the 64-1 print patterns. After that run copy auto image calibration (in copy function settings).Thankyou for mentioning the 64-5. Use a light and make sure there isn't a thin layer of dust on them. I'd give the optical mirrors under the scan glass another cleaning. Judging from the darkness of the test chart (and expected life of drum and dv), I think the problem is in the scanner (if not thin paper) and not a PM issue. After that run copy auto image calibration (in copy function settings). This machine has a built-in test chart (sim 64-5) you can print - is that faded too? It's strange that the problem area is opposite in your two samples. Is your original test chart faded in the middle? That black "100" box should be darker. ![]() The test chart looks nice and dark on the top and bottom (unlike the invoice), but possibly faded in the middle. I'm theorizing that those invoices are printed on thinner paper and that that could be affecting the scan. You can make it the default in copy function settings > initial status settings. To clarify, is the issue with copies or prints? Your wording is somewhat unclear.įor an easy/cheating solution you could scan/copy in Light Original exposure mode.
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